Objective
Despite its long history and importance for combining desirable traits such as yield, stress resistance and fruit quality, plant grafting is still restricted to a few closely related species. To overcome the cellular and molecular barriers underlying graft failure, termed incompatibility, we need an in-depth understanding of how cells at the graft interface communicate to coordinate their fusion and regeneration into a successful graft. Together with recent reports of vast formation of plasmodesmata (PD), nanoscale channels that mediate exchange of molecules and signaling cues between plant cells, my preliminary data of dynamic callose accumulation at the graft interface in Arabidopsis hypocotyls leads me to suggest that PD-mediated cell-to-cell communication is key for graft success.
However, to date, the nanoscale of PD structures and necessity of capturing transcriptional changes at single cell resolution cause methodological challenges that preclude PD’s precise roles in grafting. Meeting these challenges, I will in PD-GRAFT acquire the skill-set needed for integrating super-resolution microscopy and single-cell RNA sequencing to decipher the role of PD-mediated communication during grafting, and use this knowledge to improve grafting success. To do so, I will join Prof. Melnyk’s lab (host) and engage in two secondments to complement my current expertise in PD regulation, including grafting, regeneration, single-cell transcriptomics and PD ultrastructural imaging. While boosting my career as an independent researcher, this will allow me to provide novel insights into the molecular regulation of grafting and regeneration, specifically revealing the spatiotemporal dynamics, molecular regulators and functional roles of PD during grafting. By pinpointing key candidates, PD-GRAFT will further guide novel solutions for improving graft compatibility in agriculture and horticulture, in support of the European Grean Deal and EU’s priorities for sustainable development.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- humanities history and archaeology history
- agricultural sciences agriculture, forestry, and fisheries agriculture horticulture fruit growing
- natural sciences physical sciences optics microscopy super resolution microscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2025-PF
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
750 07 Uppsala
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.