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WhiteBrain: Decoding virus-induced brain signals in whiteflies using single-nucleus transcriptomics and functional genomics to understand behavioural manipulation leading to virus transmission

Objective

Plant viruses transmitted by insect vectors are a major threat to food security, with tomato yellow leaf curl virus (TYLCV) among the most damaging, causing up to 80% yield losses in tomato. Transmitted exclusively by the whitefly Bemisia tabaci, TYLCV manipulates both host plants and vectors to enhance its spread. Infected whiteflies display altered behaviours, including impaired host preference and prolonged feeding, but the molecular and neural mechanisms underlying these changes remain unknown. Current bulk transcriptomic approaches lack the resolution to identify the specific brain cell types and pathways driving virus-induced behavioural reprogramming.

This project pioneers the use of single-nucleus RNA sequencing (snRNA-seq) in whitefly brains to resolve virus–vector interactions at great resolution. It will (1) map cell-specific transcriptional responses to TYLCV; (2) validate candidate genes and pathways using RNAi and CRISPR/Cas9; and (3) cross-validate conserved mechanisms across B. tabaci biotypes. Behavioural assays will link molecular changes directly to vector behaviour, establishing causal pathways by which TYLCV manipulates its insect vector.

Expected outcomes include the first whitefly brain cell atlas, identification of virus-responsive neural regulators, and potential RNAi/CRISPR-based vector control strategies. These advances will also provide a scientific basis for sustainable, targeted pest management. Societal and gender dimensions are integral, as women farmers in tropical regions, who provide most agricultural labour, are disproportionately affected by whitefly-transmitted viruses.

Hosted at the University of Greenwich, with long-standing expertise on whitefly and virus research, the fellowship integrates several disciplines. Results will directly support EU’s Green Deal and Farm-to-Fork strategies by reducing pesticide reliance, safeguarding tomato production, and contributing to economic prosperity and safe food production.

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Programme(s)

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Topic(s)

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2025-PF

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Coordinator

UNIVERSITY OF GREENWICH
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 276 187,92
Address
PARK ROW GREENWICH OLD ROYAL NAVAL COLLEGE
SE10 9LS London
United Kingdom

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Region
London Outer London — East and North East Bexley and Greenwich
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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