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Engineered erythrocytes and erythrocyte extracellular vesicles for advanced Photodynamic and Sonodynamic therapy

Objective

ERYSO aims to develop innovative therapeutic solutions based on photodynamic (PDT), and sonodynamic (SDT) therapies using
engineered red blood cells (RBCs) and extracellular vesicles (EVs) derived from RBCs in combination with magnetothermal (MHT)
therapy. Both RBCs and RBC-derived EVs are characterized by their ability to transport hemoglobin, the body's natural oxygen carrier,
and exhibit long circulation times, providing a unique advantage for therapeutic delivery. Encapsulating photosensitizers (PSs) and
engineering these carriers for tumor targeting addresses a major limitation of existing PDT and SDT solutions, hypoxic tumor regions, by delivering oxygen directly to these areas, enhancing therapy efficacy. Combining PDT and SDT with MHT further amplifies
therapeutic outcomes, providing a multimodal approach to tumor eradication.
Magnetic nanoparticles for MHT or titanium oxide nanoparticles for SDT will be encapsulated in the carriers to enable combination of
therapies. Tumor targeting will be achieved by functionalizing RBC glycocalyx with targeting peptides or incorporating functionalized
lipids into EVs. Enhanced tumor uptake of EVs and RBCs will be facilitated through the Focus Ultrasound (FUS) technique, which
temporarily permeabilizes the blood-tumor barrier. Tumor targeting and oxygen delivery will be monitored using advanced
molecular imaging techniques such as Positron Emission Tomography (PET) and Single Photon Emission Computed Tomography
(SPECT). Therapeutic efficacy will be evaluated in relevant tumor models, combining molecular imaging and histopathological
analysis.
The project envisions developing personalized therapies based on autologous patient components, ensuring localized treatment
with reduced side effects. By leveraging the natural compatibility of RBCs and EVs and integrating cutting-edge targeting and delivery
technologies, ERYSO advances the potential of personalized and multimodal cancer therapies.

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Topic(s)

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Funding Scheme

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HORIZON-TMA-MSCA-SE - HORIZON TMA MSCA Staff Exchanges

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Call for proposal

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(opens in new window) HORIZON-MSCA-2025-SE-01

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Coordinator

ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 946 890,00
Address
PASEO MIRAMON 182, PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C
20009 San Sebastian
Spain

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Region
Noreste País Vasco Gipuzkoa
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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Participants (6)

Partners (7)

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