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Ultra-Scalable Genetic Programming for NAM-Ready Engineered Myotissues

Objective

SCALE-MYO will enable the large-scale deployment of New Approach Methodologies (NAM) for a tissue whose mass approaches half of one’s body weight and is one of the strongest predictors of healthy ageing: skeletal muscle. The project removes a key bottleneck to developing drugs in the multi-billion metabolic and neuromuscular disease markets and for predictive muscle toxicology: reliable and scalable supply of high-function human skeletal muscle tissues, standardised for use across quantitative assay formats. Our disruptive angle is to industrialise muscle NAM by making the cell input truly manufacturable. We will advance GERALT, a patent-pending hiPSC genetic-programming platform that strips out growth-factor dependencies, the hidden drivers of cost, variability and fragility. The result is ultra-robust cells that expand at manufacturing scale in high-density 3D and are then synchronously driven to myogenesis in just 4 days. Thaw-and-cast inputs are directly assembled into an industry-validated microphysiological system (MPS), delivering standardised engineered muscle tissues (EMT) for force and metabolic readouts within ~2 weeks. Phase 1 delivers a decision-grade package: a locked manufacturing and QC workflow, a standardised MPS assay with an analysis pipeline that has been replicated independently, and an end-user-shaped perturbation benchmark that sets pragmatic adoption thresholds (dynamic range, variability budgets, and throughput expectations), supported by targeted signalling and atrophy markers for interpretability. Adoption is executed via an adopter stack (decision, execution, distribution) with explicit gates for QC readiness, platform transfer, pharma acceptance criteria, validation/standardisation and exploitation planning. By month 9, SCALE-MYO will be ready to trigger Phase 2 scale-up and broad muscle NAM deployment, synergising with EIC portfolio projects to unlock shared standards, interoperable datasets, and faster NAM uptake across disease areas.

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HORIZON-EIC - HORIZON EIC Grants

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Call for proposal

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(opens in new window) HORIZON-EIC-2026-AIC

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Coordinator

UNIVERSITA DEGLI STUDI DI TORINO
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 300 000,00
Address
VIA GIUSEPPE VERDI 8
10124 TORINO
Italy

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Region
Nord-Ovest Piemonte Torino
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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