Objetivo The objective of this research is to gain deeper insight into how enzymes bring about catalysis of two central bioreactions, phosphate and sulphate transfer. We will use directed evolution approaches to find molecular solutions to travel the evolutionary distance between these two mechanistically related reactions. The choice of reactions is triggered by the observation of catalytic promiscuity, i.e. an enzyme activity in addition to the one it is evolved for and a possible starting point for natural evolution. In this way we attempt to mimic the way nature may have evolved new function. Kinetic and mechanistic experiments (in particular transient, pre-steady state kinetics) will enable us to trace the structural and quantitative mechanistic basis for the acquisition of a new catalytic activity. Ámbito científico ciencias naturalesciencias químicascatálisisciencias naturalesciencias biológicasbioquímicabiomoléculasproteínasenzima Palabras clave enzyme catalysis enzymology mechanism directed evolution phosphatase sulfatase Programa(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Tema(s) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Convocatoria de propuestas FP6-2002-MOBILITY-5 Consulte otros proyectos de esta convocatoria Régimen de financiación IIF - Marie Curie actions-Incoming International Fellowships Coordinador THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Dirección The old schools, trinity lane Cambridge Reino Unido Ver en el mapa Enlaces Sitio web Opens in new window Aportación de la UE Sin datos
