Periodic Reporting for period 2 - FILODIAG (Ultra-Fast Molecular Filovirus Diagnostics - Sofia ref.: 115844)
Período documentado: 2016-01-01 hasta 2017-12-31
GNA developed a protocol for the lyophilisation of the reagents to enable a ready-to-use Laser PCR mix with a shelf-life of 2 months at room temperature, or even longer when cooled. A one-step RT-Laser PCR duplex assay for the Ebola + IC (internal process control) targets was developed and optimized by GNA, and successfully tested by INMI and partners in the field in Sierra Leone on ribonucleic acids isolated from diverse samples (plasma, urine, and oral), derived from Ebola-infected patients from the 2014-2015 outbreak.
The Laser PCR technology, subject to this project, is now already commercially available through GNA Biosolutions GmbH. The experiences with the Pharos400 prototype, with its Laser PCR amplification and fluorescence-based detection, laid the foundation for developing and launching an industrialized laboratory instrument, the Pharos V8, after this project.
Up to now, the project has already generated a patent application, articles, and posters at international scientific meetings.