Objective This project is aimed at a better understanding, diagnosis and treatment orientation of allergy, a disease undergoing explosive and worrisome progress. The team will design a new, miniature and automated multicriteria platform for separating allergens from food and/or environmental samples, and for detecting their allergenic activities on patient antibodies immobilised on anti-IgE and anti IgG4 strips. The project proposes a dramatic renewal of protein separation conventional schemes, by combining isoelectr ic focusing with a novel second dimension electrophoresis preserving the native, active structure of the proteins. This paradigmatic change will be associated with the power of newly developed microfluidic (lab-on-chip) systems, and with a new sensitive el ectrochemical method for the measurement of label free affinity sensograms, to allow automated and quantitative multicriteria screening. In contrast with current screening methods for allergy, which search for the response of a patient's serum to a limite d predefined set of known allergens, the approach developed in this project aims at screening the COMPLETE SET of potential allergens contained in a whole food extract (or more generally in any sample relevant to the patient's environment) against the pati ent's IgE and IgG4, WITHOUT ANY A PRIORI LIMITATION, and to identify the nature of the captured immunogens. Thus, besides strong innovation in the methodology of protein separation, the project proposes a radical enhancement in the power of allergen screen ing, with considerable potential for research, diagnosis and treatment follow-up of allergy. Fields of science engineering and technologyother engineering and technologiesmicrotechnologylab on a chipnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesclinical medicineallergologynatural scienceschemical scienceselectrochemistryelectrophoresis Keywords 2D separations allergen detection and profiling carrier ampholyte based capillary electrophoresis label-free electrochemical detection Programme(s) FP6-POLICIES - Policy support: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) NEST-2003-1 - Adventure activities Call for proposal FP6-2003-NEST-B-1 See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE Address Lausanne Switzerland See on map Links Website Opens in new window EU contribution € 0,00 Participants (3) Sort alphabetically Sort by EU Contribution Expand all Collapse all CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE France EU contribution € 0,00 Address Rue michel-ange, 3 Paris See on map Links Website Opens in new window INSTITUT CURIE France EU contribution € 0,00 Address 26, rue d'ulm Paris See on map Links Website Opens in new window POLITECNICO DI MILANO Italy EU contribution € 0,00 Address Piazza leonardo da vinci 32 Milano See on map Links Website Opens in new window
