Objective
There is an urgent clinical requirement for appropriate bone substitutes that are able to replace current autologous and allogeneic grafting procedures for the repair of diseased or damaged skeletal tissues. Mesenchymal stem cells (MSCs), found predominantly in the bone marrow, are able to differentiate into osteogenic, chondrogenic, adipogenic and tenogenic lineages, thus offering considerable therapeutic potential for tissue engineering applications. However, invasive extraction procedures and insufficient viable cell yields have necessitated the identification of alternative tissue sources of MSCs. Growing evidence suggests that umbilical cord blood (UCB) contains a population of rare MSCs that are able to undergo multilineage differentiation.
The aim of this proposal is optimise the isolation and expansion of MSCs from human UCB (CB-MSCs). The differentiation capacity of CB-MSCs will be examined, with a specific focus on osteogenesis. The CB-MSCs will be characterised by genomic, proteomic and bioimpedance profiling and compared to MSCs isolated from human bone marrow as well as embryonic stem cells. Full bioinformatics integration of datasets will identify specific and/or novel signalling factors associated with CB-MSCs. The immunophenotype and alloreactivity of CB-MSCs will be determined. Comparative analyses of the population doubling times, telomere length and telomerase activity will identify the lifespan of CB-MSCs. Novel expansion techniques will be combined with scale-up procedures and the generation of CB-MSC lines for banking using optimised cryopreservation protocols. In vitro and in vivo biocompatibility assays using a range of biomimetic scaffolds will be exploited, complementing in vivo homing and engraftment models. Our integrated approach using complementary expertise will provide a timely and thorough evaluation of CB-MSCs and define appropriate routes for their therapeutic implementation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine surgery
- medical and health sciences medical biotechnology tissue engineering
- medical and health sciences basic medicine immunology
- medical and health sciences medical biotechnology cells technologies stem cells
- medical and health sciences clinical medicine transplantation
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-LIFESCIHEALTH-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
YORK
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.