Objective With each passing year since Chernobyl accident, more questions arise about the potential for organisms adapt to radiation exposure. The explosion of one of the four reactors of Chernobyl nuclear power plant on 26 April 1986 caused worst environmental nuclear disaster in the history. It transported vast amounts of radioactive material into the atmosphere, much of which was subsequently deposited not only in the immediate vicinity of power plant in Ukraine, Belarus and Russia, but over the large parts of Europe. In this proposal, quantitative protein reference maps will be used to identify differentially expressed proteins in developing embryo, endosperm and seed coats of Chernobyl-grown plants compared to control. These reference maps will be generated during five stages of soybean and flax seed development using two-dimensional electrophoresis (2-DE) in combination with liquid chromatography connected with tandem mass spectrometry (LC-MS/MS) for protein identification. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsengineering and technologyother engineering and technologiesnuclear engineeringnatural scienceschemical scienceselectrochemistryelectrophoresisnatural scienceschemical sciencesanalytical chemistrymass spectrometrymedical and health sciencesclinical medicineembryology Keywords Natural sciences Plant development Proteomics Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) PEOPLE-2007-4-3.IRG - Marie Curie Action: "International Reintegration Grants" Call for proposal FP7-PEOPLE-2007-4-3-IRG See other projects for this call Funding Scheme MC-IRG - International Re-integration Grants (IRG) Coordinator INSTITUTE OF PLANT GENETICS AND BIOTECHNOLOGY EU contribution € 100 000,00 Address Akademická 95007 NITRA Slovakia See on map Activity type Higher or Secondary Education Establishments Administrative Contact Anna Pret'ová (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
