With each passing year since Chernobyl accident, more questions arise about the potential for organisms adapt to radiation exposure. The explosion of one of the four reactors of Chernobyl nuclear power plant on 26 April 1986 caused worst environmental nuclear disaster in the history. It transported vast amounts of radioactive material into the atmosphere, much of which was subsequently deposited not only in the immediate vicinity of power plant in Ukraine, Belarus and Russia, but over the large parts of Europe. In this proposal, quantitative protein reference maps will be used to identify differentially expressed proteins in developing embryo, endosperm and seed coats of Chernobyl-grown plants compared to control. These reference maps will be generated during five stages of soybean and flax seed development using two-dimensional electrophoresis (2-DE) in combination with liquid chromatography connected with tandem mass spectrometry (LC-MS/MS) for protein identification.
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