Skip to main content

Development of methodology for alternative testing strategies for the assessment of the toxicological profile of nanoparticles used in medical diagnostics

Objective

Nanoparticles (NP) have unique, potentially beneficial properties, but their possible impact on human health has not been adequately assessed. The main goal of this proposal is to develop alternative high-throughput testing strategies using in vitro and in silico methods to assess the toxicological profile of NP used in medical diagnostics. Our specific aims are to: 1. Define NP properties and fully characterize NP to be used 2. Study NP interactions with molecules, cells and organs and develop in vitro methods to study the toxicological potential of NP 3. Validate in vitro findings in short-term in vivo models and study particle effects in animals and (ex vivo) in humans to assess individual susceptibility to NP 4. Develop in silico models of NP interactions Experimental work is structured in 4 WPs to address NP characterisation and key elements in evaluation of NP uptake, exposure and toxicology. NANOTEST integrates the investigation of toxicological properties and effects of NP in several target systems by developing a battery of in vitro assays using cell cultures, organotypic cell culture and small organ fragments (ex vivo) derived from different biological systems; blood, vascular system, liver, lung, placenta, digestive and central nervous systems. As NP action is likely to involve oxidative stress we will focus on the cross-cutting areas of inflammation, cellular toxicity, immunotoxicity, genotoxicity and related endpoints. Following development of SOP and generation of a common database and in parallel with in silico assays (QSAR, PBPK modelling), NanoTest will evaluate toxic effects and interactions of NP used in nanomedicine. Results will be validated in an experimental ethically approved in vivo model. The most advanced and standardised techniques will be adapted for automation and prepared for validation by ECVAM. Separate WPs will be dedicated to dissemination and to effective administrative and scientific management.

Field of science

  • /social sciences/sociology/industrial relations/automation
  • /medical and health sciences/medical biotechnology/nanomedicine
  • /medical and health sciences/basic medicine/toxicology

Call for proposal

FP7-HEALTH-2007-A
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

NORSK INSTITUTT FOR LUFTFORSKNING STIFTELSE
Address
Instituttveien 18
2027 Kjeller
Norway
Activity type
Research Organisations
EU contribution
€ 578 430
Administrative Contact
Maria Dusinska (Dr.)

Participants (11)

INSTITUTE OF OCCUPATIONAL MEDICINE
United Kingdom
EU contribution
€ 130 593
Address
Research Avenue North, Riccarton 45
EH14 4AP Edinburgh
Activity type
Research Organisations
Administrative Contact
Susy Scarisbrick (Ms.)
CENTRE HOSPITALIER UNIVERSITAIRE VAUDOIS
Switzerland
EU contribution
€ 215 750
Address
Rue Du Bugnon 21
1011 Lausanne
Activity type
Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments)
Administrative Contact
Bernard Gmünder (Mr.)
UNIVERSITE PARIS DIDEROT - PARIS 7
France
EU contribution
€ 193 250
Address
Rue Thomas Mann 5
75205 Paris
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Muriel Maurice (Ms.)
University Hospitals Bristol NHS Foundation Trust
United Kingdom
EU contribution
€ 188 196
Address
Trust Headquarters, Marlborough Street
BS13NU Bristol
Activity type
Research Organisations
Administrative Contact
Margaret Saunders (Dr.)
JRC -JOINT RESEARCH CENTRE- EUROPEAN COMMISSION
Belgium
EU contribution
€ 463 842
Address
Rue De La Loi 200
1049 Brussels
Activity type
Research Organisations
Administrative Contact
Raymond Crandon (Mr.)
"NATIONAL CENTER FOR SCIENTIFIC RESEARCH ""DEMOKRITOS"""
Greece
EU contribution
€ 150 050
Address
End Of Patriarchou Grigoriou E And 27 Neapoleos Street
15341 Agia Paraskevi
Activity type
Research Organisations
Administrative Contact
Stella Martaki (Ms.)
SLOVENSKA ZDRAVOTNICKA UNIVERZITA V BRATISLAVE
Slovakia
EU contribution
€ 339 750
Address
Limbova 12
83303 Bratislava
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Milan Cagáň (Mr.)
KOBENHAVNS UNIVERSITET
Denmark
EU contribution
€ 188 670
Address
Norregade 10
1165 Kobenhavn
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ivan Kristoffersen (Mr.)
ADVANCELL ADVANCED IN VITRO CELL TECHNOLOGIES S.A.
Spain
EU contribution
€ 308 508,99
Address
C/baldiri Reixac 10-12
08028 Barcelona
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Luis Ruiz Avila (Dr.)
UNIVERSITA CA' FOSCARI VENEZIA
Italy
EU contribution
€ 158 520
Address
Dorsoduro 3246
30123 Venezia
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Emanuele Argese (Mr.)
ACONDICIONAMIENTO TARRASENSE ASSOCIACION
Spain
EU contribution
€ 78 823,01
Address
Carrer De La Innovacio 2
08225 Terrassa
Activity type
Research Organisations
Administrative Contact
Izabel Alfany (Dr.)