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MICROARRAYS FOR THE DETECTION OF TOXIC ALGAE

Objective

Microalgae in marine and brackish waters of Europe regularly cause «harmful effects», considered from the human perspective, in that they threaten public health and cause economic damage to fisheries and tourism. Cyanobacteria cause similar problems in freshwaters. These episodes encompass a broad range of phenomena collectively referred to as «harmful algal blooms» (HABs). They include discoloration of waters by mass occurrences of microalgae (true algal blooms that may or may not be «harmful») to toxin-producing species that may be harmful even in low cell concentrations. A broad classification of HAB distinguishes three groups of toxic organisms. For adequate management of these phenomena, monitoring of microalgae is required. However, the effectiveness of monitoring programmes is limited by the fact that it is time consuming and morphology as determined by light microscopy may be insufficient to give definitive species and toxin attribution. Once cell numbers reach a threshold level, then shellfish are selected to toxin analysis by the mouse bioassay. The mouse bioassay is continued on a daily basis until no more toxin is detected. Molecular and biochemical methods are now available that offer rapid means of both species and toxin detection. In this project we will target rapid species identification using rRNA genes as the target. We include antibodies to specific toxins because even when cell numbers are very low, the toxins can be present and can be accumulated in the shellfish. Microarrays are the state of the art technology in molecular biology for the processing of bulk samples for detection of target RNA/DNA sequences.. The purpose of MIDTAL is to support the common fisheries policy to aid the national monitoring agencies by providing new rapid tools for the identification of toxic algae and their toxins so that they can comply with ECC directive 91/1491/CEE that can be converted to cell numbers and reduce the need for the mouse bioassay.

Field of science

  • /medical and health sciences/health sciences/public and environmental health
  • /agricultural sciences/agriculture, forestry, and fisheries/fisheries
  • /natural sciences/biological sciences/molecular biology
  • /agricultural sciences/agriculture, forestry, and fisheries
  • /natural sciences/biological sciences/microbiology/phycology

Call for proposal

FP7-ENV-2007-1
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM
Address
Citadel Hill The Laboratory
PL1 2PB Plymouth
United Kingdom
Activity type
Research Organisations
EU contribution
€ 253 678
Administrative Contact
Parr Jon (Mr.)

Participants (10)

STAZIONE ZOOLOGICA ANTON DOHRN
Italy
EU contribution
€ 316 078
Address
Villa Comunale
80121 Napoli
Activity type
Research Organisations
Administrative Contact
Wiebe Kooistra (Dr.)
LINNEUNIVERSITETET
Sweden
EU contribution
€ 323 370
Address
Linnaeus University
35195 Vaxjo
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Anna Gustavsson (Ms.)
INSTITUTO ESPANOL DE OCEANOGRAFIA
Spain
EU contribution
€ 248 879
Address
Corazon De Maria 8
28002 Madrid
Activity type
Research Organisations
Administrative Contact
Enrique Tortosa (Dr.)
NATIONAL UNIVERSITY OF IRELAND GALWAY
Ireland
EU contribution
€ 248 379
Address
University Road
H91 Galway
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Daniel O'mahony (Dr.)
UNIVERSITETET I OSLO
Norway
EU contribution
€ 296 353
Address
Problemveien 5-7
0313 Oslo
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Tone Tellevik Dahl Dahl (Ms.)
THE UNIVERSITY OF WESTMINSTER LBG
United Kingdom
EU contribution
€ 252 295
Address
Regent Street 309
W1B 2UW London
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jane Lewis (Prof.)
DHI

Participation ended

Denmark
EU contribution
€ 12 181,59
Address
Agern Alle 5
2970 Horsholm
Activity type
Research Organisations
Administrative Contact
Hanne Kaas (Dr.)
INSTITUTO TECNOLOXICO PARA O CONTROL DOMEDIO MARINO DE GALICIA - INTECMAR
Spain
EU contribution
€ 154 922
Address
Peirao De Villaxoan S/n
36611 Villagarcia De Arousa
Activity type
Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments)
Administrative Contact
Xosé Manoel Romarís (Mr.)
THE UNIVERSITY OF RHODE ISLAND
United States
Address
Lower College Road 70
02881 Kingston
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Lucie Maranda (Dr.)
THE QUEEN'S UNIVERSITY OF BELFAST
United Kingdom
EU contribution
€ 128 714,41
Address
University Road Lanyon Building
BT7 1NN Belfast
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Chris Elliott (Prof.)