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Effective redesign of oxidative enzymes for green chemistry

Objective

Enzymes are extremely powerful natural catalysts able to perform almost any type of chemical reaction while being mild by nature and highly specific. In fact, the delicate functioning of enzymes forms the basis of every living creature. The catalytic potential of enzymes is more and more appreciated by the industry as many industrial processes rely on these sophisticated catalysts. However, the number of reactions catalyzed by enzymes is restricted as enzymes only have evolved to catalyze reactions that are physiologically relevant. Furthermore, enzymes have adapted to the direct (cellular) environment in which they have to function (e.g. operative at ambient temperature, resilient towards proteolysis, catalytic turnover rate should fit with metabolic enzyme partners). This excludes the existence of enzymes that do not fit within boundaries set by nature. It is a great challenge to go beyond these natural boundaries and develop methodologies to design ‘unnatural’ tailor-made enzymes. Ideally it should become possible to (re)design enzymes to convert pre-defined substrates. Such designer enzymes could theoretically exhibit unsurpassed catalytic properties and, obviously, will be of significant interest for industrial biotechnology. The OXYGREEN project aims at the design and construction of novel oxygenating enzymes (designer oxygenases) for the production of compounds that can be used in medicine, food and agriculture and the development of novel powerful and generic enzyme redesign tools for this purpose. The enzymes and whole-cell biocatalysts that will be developed should catalyze the specific incorporation of oxygen to afford synthesis of bioactive compounds in a selective and clean way, with minimal side products and with no use of toxic materials. For this, generic platform technologies (novel high-throughput methodology and methods for engineering dedicated host cells) will be developed that allow effective structure-inspired directed evolution of enzyme.

Field of science

  • /engineering and technology/industrial biotechnology
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes

Call for proposal

FP7-KBBE-2007-1
See other projects for this call

Funding Scheme

CP-IP - Large-scale integrating project

Coordinator

RIJKSUNIVERSITEIT GRONINGEN
Address
Broerstraat 5
9712CP Groningen
Netherlands
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 203 149
Administrative Contact
Jan Poutsma (Mr.)

Participants (13)

JACOBS UNIVERSITY BREMEN GGMBH

Participation ended

Germany
EU contribution
€ 121 989
Address
Campus Ring 1
28759 Bremen
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ronald Kieschnick (Dr.)
TECHNISCHE UNIVERSITAT DORTMUND
Germany
EU contribution
€ 882 950
Address
August Schmidt Strasse 4
44227 Dortmund
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Detlef Niehage (Mr.)
BIOINFOBANK INSTITUTE
Poland
EU contribution
€ 409 200
Address
Limanowskiego 24/A 16
60744 Poznan
Activity type
Research Organisations
Administrative Contact
Jadwiga Borowcyk (Dr.)
TECHNISCHE UNIVERSITAET WIEN
Austria
EU contribution
€ 363 600
Address
Karlsplatz 13
1040 Wien
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Marko Mihovilovic (Prof.)
TECHNISCHE UNIVERSITAET GRAZ
Austria
EU contribution
€ 832 013
Address
Rechbauerstrasse 12
8010 Graz
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Anton Glieder (Prof.)
UNIVERSITA DEGLI STUDI DI PAVIA
Italy
EU contribution
€ 373 350
Address
Strada Nuova 65
27100 Pavia
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Andrea Mattevi (Prof.)
DSM RESEARCH B.V.
Netherlands
EU contribution
€ 11 892
Address
Urmonderbaan 22
6167RD Geleen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Johan H.M. Jansen (Dr.)
EVONIK REXIM
France
EU contribution
€ 91 528
Address
Rue De Verdun 33
80400 Ham
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Dominique Frossard (Dr.)
BIOLOG LSI FORSCHUNGS- UND BETEILIGUNGS-GMBH
Germany
EU contribution
€ 258 400
Address
Flughafendamm 9A
28199 Bremen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Hans-Gottfried Genieser (Dr.)
ENZYSCREEN
Netherlands
EU contribution
€ 98 400
Address
Wassenaarseweg
2333 AL Leiden
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Wouter Duetz (Dr.)
DECHEMA GESELLSCHAFT FUER CHEMISCHE TECHNIK UND BIOTECHNOLOGIE E.V.
Germany
EU contribution
€ 250 140
Address
Theodor Heuss Allee 25
60486 Frankfurt
Activity type
Research Organisations
Administrative Contact
Jens Schrader (Dr.)
RHEINISCH-WESTFAELISCHE TECHNISCHE HOCHSCHULE AACHEN
Germany
EU contribution
€ 578 530
Address
Templergraben 55
52062 Aachen
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ernst Schmachtenberg (Prof.)
DSM INNOVATIVE SYNTHESIS BV
Netherlands
EU contribution
€ 68 108
Address
Poststraat 1
6135 KR Sittard
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Ine Weijer Van De (Mrs.)