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Coupling chip technology and affinity capillary electrophoresis for a faster, cheaper and high-throughput analysis of biological systems

Objectif

Microfluidic 'lab-on-a-chip' technology has revolutionized laboratory experimentation, thereby, demonstrating the potential of miniaturization, integration, and automatization to research-based industries.

There is also a great need in the area of drug de sign and development to develop analytical techniques that require small amounts of material and which can analyze samples expeditiously and provide accurate measurement of physicochemical parameters. The proposed research is unique and innovative in that it combines state-of-the-art microfluidic and capillary electrophoresis (CE) separation techniques with molecular derivatization.

Although CE on a microchip is relatively well-established the coupling of it to molecular derivatization reactions has only recently been examined and only in the Gomez laboratories. The chip electrophoresis technology needs mainly microfluidic and electromigration research.

The applicant has a basic knowledge on both fields, however, he is not familiar in CE on a chip, and he cannot acquire this expertise in Hungary. At this time the best place to obtain this knowledge is in California (United States) in the labs of Professor Frank A. Gomez, where both chip electrophoresis technology and affinity CE can be studied.

The applicant hopes to transfer the cutting-edge chip-CE technology from the United States to a less-favoured, eastern region of EU. The research will develop analytical techniques including design, preparation, and application of microfluidic devices and CE and will focus on examining molecules and enzymes involved in disease and public health.

To create 'lab-on-a-chip' devices, manufacturing methods from the microchip industry were coupled to techniques involving fluid dynamics, analytical chemistry, and biochemistry resulting in miniature integrated biochemical processing systems. Hence, the proposed work described, herein, should be considered interdisciplinary and multidisciplinary in nature.

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Appel à propositions

FP6-2004-MOBILITY-6
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Coordinateur

UNIVERSITY OF DEBRECEN, SCHOOL OF INDEPENDENT FACULTIES
Contribution de l’UE
Aucune donnée
Coût total
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Participants (1)