Objective
Smal RNAs and their role in posttranscriptional silencing is the latest fascinating paradigm in molecular biology. In response to virus infection, transposons and transgenes, an "adaptive immune-system" is induced based on recognition of double-stranded (ds)RNA, excessive quantities of RNA and yet undefined abnormal properties of RNA. dsRNA is recognized by ""Dicer"", an RNAseIII-like enzyme and "diced" into classes of "small interfering RNAs", 21 to 25 nucleotides in length. These original double-stranded fragments are unwound by a helicase and one of the single strands builds an ""RNA-induced silencing complex"" (RISC). RISC and related complexes have various functions. They interact with and cleave target RNAs.
They can interact with target RNAs and produce new dsRNA with the siRNA as a primer and RdRp as an enzyme. In the nucleus they can scan the chromatin for cognate DNA and modify it by RNA dependent DNA methylation. Silencing in plants is so successful, that plant viruses cannot infect a plant without a counter defence mechanism. Depending on the virus, silencing suppression can be a secondary function of almost any type of viral protein. But little is known about the silencing suppression mechanisms.
Of special interest might be the silencing mechanism interfering with Gemini-viruses and the suppression tactics of those viruses, since in that cases a nuclear phase in virus replication has to be considered. Gemini-viruses are economically important pathogenes. The host lab has found that in Gemini-viruses the transcriptional transactivator AC2 acts as a silencing suppressor and that this probably occurs through the activation of host genes. Wheat germ (WG) extracts are widely used to study translation regulation. WG extract contains silencing activities. We are planning to use WG and protoplasts extracts to study molecular aspects of silencing and its suppression, especially when induced and controlled by Gemini-viruses.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences biological sciences molecular biology
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-7
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
BASEL
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.