Objective The development of high resolution, non damaging imaging techniques are crucial for understanding the biological processes occurring at the cellular level. Nonlinear microscopy (NLM) is rapidly establishing as a powerful technique for high resolution imaging of living biological samples. The high peak powers and low pulse energies available from ultrashort pulses, allow an efficient excitation of nonlinear effects with reduced collateral damage when interacting with cells. Ultrashort pulse light has three additional parameters that can be exploited for a more efficient light-cell interaction. i) Pulse shaping, acting on the temporal intensity profile and phase parameters of a pulse will allow for a more efficient and less damaging interaction with the sample. ii) Adaptive optics will be used to modulate the beam’s wavefront spatial distribution parameter. This will allow correcting aberrations for an increased transversal resolution, larger penetration depths and fields of view. As focus spot size is reduced, it will also allow for lower power to be used, preserving living specimens. In this project, we aim, for the first time, to join complementary intersectorial expertise to perform simultaneous pulse shaping and wavefront correction at the sample plane of a NLM. The knowledge of each of the teams will be transferred, based on microscopy applications. Firstly the work will be focused towards implementing adaptive optics to minimise aberrations induced by the setup and by the sample of study. Then pulse shaping will be use to enhance the output signal and reduce side effects (such as photobleaching). Finally, the combined action will result on new contrast generation technique, constituting an important breakthrough in NLM with great implications into bio-medicine. Fields of science natural sciencesphysical sciencesopticsmicroscopy Keywords Applied optics Instrumentation technology Laser technology Microscopy Physical sciences Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) PEOPLE-2007-3-1-IAPP - Marie Curie Action: "Industry-Academia Partnerships and Pathways" Call for proposal FP7-PEOPLE-2007-3-1-IAPP See other projects for this call Funding Scheme MC-IAPP - Industry-Academia Partnerships and Pathways (IAPP) Coordinator FUNDACIO INSTITUT DE CIENCIES FOTONIQUES EU contribution € 275 735,00 Address AVINGUDA CARL FRIEDRICH GAUSS 3 08860 Castelldefels Spain See on map Region Este Cataluña Barcelona Activity type Research Organisations Administrative Contact Dolors Mateu (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all IMAGINE OPTIC France EU contribution € 249 027,00 Address 18 RUE CHARLES DE GAULLE 91400 Orsay See on map Region Ile-de-France Ile-de-France Essonne Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Nathalie Mandonnet (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
