Objective Although our knowledge about the function of basic biological elements such as genes and proteins has grown considerably, we lack a comprehensive picture of how these elements act in coordination, and our ability to modulate their function is still limited. Here, I propose a global study of motif-mediated protein interactions that aims to find interactions that can be modulated by small molecules or peptides, and their roles in the organization of signaling pathways. I will use one of the best-understood eukaryotic model organism Saccharomyces cerevisiae, for which several large-scale functional, interaction and chemical-genetic datasets are available. In addition, the genome sequences for more than a dozen of yeast species are largely complete. I will identify motif-mediated interactions using these proteomic and comparative data, and integrate them with large-scale chemical-genetic datasets to explore the correlations between genetic interactions and small molecules. I will test my predictions and study cellular pathways cross-talk using modelling and combinatorial protein interaction interference. With such a systematic approach, I aim to reveal the fundamental principles of how protein function can be modulated, and how these principles might carry over to vertebrates. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsgenomes Keywords Saccharomyces cerevisiae chemical-genetics interaction modulation protein linear motifs protein-protein interactions signal transduction pathways Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) PEOPLE-2007-2-1.IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Call for proposal FP7-PEOPLE-2007-2-1-IEF See other projects for this call Funding Scheme MC-IEF - Intra-European Fellowships (IEF) Coordinator THE UNIVERSITY OF EDINBURGH Address Old college, south bridge EH8 9YL Edinburgh United Kingdom See on map Region Scotland Eastern Scotland Edinburgh Activity type Higher or Secondary Education Establishments Administrative Contact Angela Noble (Ms.) Links Contact the organisation Opens in new window Website Opens in new window EU contribution € 178 307,05
