Objective
Filamentous bacteriophages comprise a family of viruses that share a similar virion structure and life cycle. The virion is composed of a circular ssDNA wrapped by thousands of similar copies of a major coat protein and several different minor coat proteins in both ends. Due to the mass (tens of MegaDaltons) and dimensions (~7 nm in diameter, 600 – 2000 nm in length) of filamentous phages, structural models have been obtained primarily from fiber diffraction, and from static solid state NMR of aligned concentrated solutions. Yet, these models are incomplete, and vary even within individual virions. Also, some key questions regarding DNA structure, subunit-DNA interactions, microscopic polymorphism, and more, remain unclear or are in debate. Magic-angle spinning solid state NMR (MAS SSNMR) has been shown recently to be a complementary and successful method for studying the structure and dynamics of biological macromolecules. Examples are membrane proteins, protein aggregates and fibrils, and recently, the Pf1 filamentous phage. Also, sample preparation for MAS SSNMR is more flexible, and measurements can be performed in conditions, which are either biologically relevant, or important for their biophysical characterization. The goal of the proposed project is to structurally characterize and compare the three key phages fd, Ike, and Pf3, using MAS SSNMR as a principal spectroscopic tool. These phages were chosen, since they represent different structural properties of such virions. We anticipate that our proposed studies will resolve some of the key questions regarding the structural assembly of filamentous phages, and enhance our understanding on the possible ways in which these phages assemble at their host bacteria. Our results will also provide suitable model systems for studies of other complex biological systems.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics spectroscopy absorption spectroscopy
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-4-3-IRG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69978 Tel Aviv
Israel
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.