Objective
SMYD3 is a histone methylase that di- and tri-methylates histone H3 at lysine 4. In normal conditions SMYD3 is highly expressed only in skeletal muscle and testis. We hypothesize that SMYD3 plays an important role in transcriptional regulation throughout myogenesis. We will use C2C12 myoblast and primary satellite cells as model systems to assess the contribution of SMYD3 to the transcriptional regulation of muscle specific genes. We will over-express and knock down SMYD3 to determine the impact of SMYD3 on the expression of myogenic markers and the ability of C2C12 cells to fuse into multinucleated myotubes. We will also determine functional mechanisms that account for a potential involvement of SMYD3 in myogenesis by employing chromatin immunoprecipitation experiments. Moreover, we will identify SMYD3 target genes in muscle cells by RNA microarray analysis and by the combination of the chromatin immunoprecipitation technique with massively sequencing technology (ChIp-seq). A further objective of the project will be to gain insights into the mechanisms underlying SMYD3 activity and its modulation, by exploiting in vitro and in vivo approaches.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology mechanical engineering vehicle engineering aerospace engineering satellite technology
- natural sciences biological sciences genetics RNA
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-4-3-IRG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
20122 Milano
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.