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Parallel fluorescence spectroscopy tools for micro and nano-analytical applications down to single biomolecules

Objective

Analytical methods based on fluorescence measurements are widely employed for investigating biological process at cellular level. A modern technique is fluorescence-lifetime imaging microscopy (FLIM), where a map is obtained of the fluorescent emission lifetime versus position in a cell. The objective of project PARAFLUO is an innovative instrumentation system that will enhance and extend the usefulness of FLIM, making possible to obtain simultaneously FLIM data separately for the various spectral components of the emission. There is wide consensus among experimenters that this spectrally resolved technique (called sFLIM) will support a better understanding of the biological processes involved. Such understanding is paramount for the (patho)physiology of tissues and organisms and gives a base for gaining a better insight in key medical issues, such as the origin and growth mechanisms of tumors. The optoelectronic instrumentation developed will be useful also for other market objectives, such as simultaneous multi-spectral profiling of objects by laser detection and ranging (LADAR) techniques. The developments envisaged are essentially: (a) a photon-counting array detector based on the silicon single-photon avalanche diode (SPAD) technology; (b) a new micro-lens system for focusing light onto the detector and (c) an ASIC based multichannel time correlated single photon counting (TCSPC) system, integrated with an optoelectronic setup in a confocal microscope. The base of the PARAFLUO consortium is given by three SME’s; each one having a consolidated technical know-how and an active presence in the market over one of the quoted scientific-technical (S/T) areas. Five RTD performers have been selected primarily because of their high international standard in these areas; furthermore, each of them has experience of active collaboration with the SME directly concerned by the specific S/T work. A professional partner supports the coordinator and ensures timely and efficient exchange of materials and information in the project.

Call for proposal

FP7-SME-2008-1
See other projects for this call

Coordinator

MICRO PHOTON DEVICES SRL
Address
Via Waltraud Gebert Deeg 3/F
39100 Bolzano
Italy
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
EU contribution
€ 543 030
Administrative Contact
Roberto Biasi (Dr.)

Participants (8)

PicoQuant GmbH
Germany
EU contribution
€ 417 009
Address
Rudower Chaussee 29
D-12489 Berlin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Rainer Erdmann (Dr.)
HELIA PHOTONICS LTD
United Kingdom
EU contribution
€ 110 251
Address
Rosebank Park 2
EH54 7EJ Livingston
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Caspar Clark (Dr.)
POLITECNICO DI MILANO
Italy
EU contribution
€ 6 380
Address
Piazza Leonardo Da Vinci 32
20133 Milano
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Massimo Ghioni (Prof.)
CONSIGLIO NAZIONALE DELLE RICERCHE
Italy
EU contribution
€ 6 431
Address
Piazzale Aldo Moro 7
00185 Roma
Activity type
Research Organisations
Administrative Contact
Piera Maccagnani (Dr.)
Fachhochschule Brandenburg
Germany
EU contribution
€ 6 300
Address
Magdeburger Str. 50
14770 Brandenburg
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Gerald Kell (Prof.)
GEORG-AUGUST-UNIVERSITAT GOTTINGEN STIFTUNG OFFENTLICHEN RECHTS
Germany
EU contribution
€ 6 300
Address
Wilhelmsplatz 1
37073 Gottingen
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Joerg Enderlein (Prof.)
HERIOT-WATT UNIVERSITY
United Kingdom
EU contribution
€ 6 299
Address
Riccarton
EH14 4AS Edinburgh
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Gerald Buller (Prof.)
CF CONSULTING FINANZIAMENTI UNIONE EUROPEA SRL
Italy
EU contribution
€ 31 000
Address
Via Giuseppe Mussi 1
20154 Milano
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Carla Finocchiaro (Mrs.)