Objective
X-inactivation (XCI) is an epigenetic phenomenon resulting in transcriptional silencing to attain gene dosage parity between XX females and XY males. XCI involves coating of the non-coding Xist RNA followed by depletion of euchromatic marks and shortly after accumulation of heterochromatic marks. The chromatin modifying activities that are involved in these steps and their functional interplay remain poorly understood however. We will specifically assess how euchromatic marks, such as H3K4 methylation and H3 and H4 acetylation, are lost shortly after Xist RNA coating and the importance of this loss in gene silencing and heterochromatin formation. First, we will perform functional studies in differentiating XX ES cells, including knockdown of chromatin modifying candidates, such as H3K4 demethylases and histone deacetylases. Second, we will investigate how specific X-linked loci behave during XCI, using a Lac or Tet operator tagging system. This will allow us to (a) visualise a locus in living cells and assess the exact timing of recruitment of proteins of interest (fused to a fluorescent protein), in control and knockdown situations; and (b) target specific chromatin factors (using the Lac or Tet repressor fusion) to a locus in order to assess the impact of chromatin changes on XCI. In summary, the two complementary strategies outlined here should provide us with new insights into the roles that various chromatin changes associated with XCI actually play in this process. This should also give new insights into the process of gene regulation in other systems.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- engineering and technology materials engineering coating and films
- natural sciences biological sciences genetics RNA
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IEF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75231 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.