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Content archived on 2024-05-29

HIGH-THROUGHPUT STRUCTURAL STUDIES OF PROKARYOTIC ACTIVE SECONDARY TRANSPORTERS

Objective

The proposed project is aiming at solving the crystallographic structure of some of the prokaryotic membrane proteins belonging to the family of Active Secondary Transporters, an important and heterogeneous class of symport/antiport systems involved in cel l homeostasis and conserved across all reigns of living organisms. Given the difficulty encountered in membrane protein production and crystallization, a High-Throughput approach will be used, in order to screen the highest possible number of different exp ression, solubilization and crystallization conditions and to enhance the probability of success for a few targets out of a starting set of about 100 cloned genes. If determining even only one structure would be an important scientific result, given the ve ry low number of membrane protein structures deposited in the Protein Data Bank, obtaining a few of them would result in a significant improvement in understanding the structural, and hence functional, differences between members of this protein family. Fu rther on, this project will lead to the optimisation of High-Throughput techniques towards membrane protein production and crystallization and will provide a large amount of data to be used for rationalizing the parameters that dictate successes and failur es in membrane protein biochemistry.

Fields of science (EuroSciVoc)

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Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP6-2004-MOBILITY-5
See other projects for this call

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

EIF - Marie Curie actions-Intra-European Fellowships

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
EU contribution
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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