Objective After a decade of development in model organisms and later in mammalian cells, mass spectrometry-based functional proteomics approaches have come of age and are ready to enable a systematic study of the innate immune system. We propose to cross the large-scale proteomics and innate immunity disciplines to obtain a functionally annotated map of the molecular machinery involved in viral recognition and leading to the hallmark interferon response, through a three-pronged approach: 1. Map the interactome of innate immunity proteins in macrophages to establish the network of components leading to interferon production; 2. Chart the interactions of molecular patterns, mostly nucleic acids, to identify the receptors and sensors at the non-self/self interface; 3. Study viral pathogenicity factors as molecular jammers of the anti-viral response and elucidate their mode of action to uncover critical nodes (inhibitome). Datasets are integrated and released at regular intervals with embargoed windows allowing a network of collaborators/own laboratory to do in-depth validation. New components at data intersections will be tested through loss-of-function experiments and standardized read-outs for the interferon pathway as well as genetic association with autoimmune diseases. Because of its unbiased/large scope and its cross-validating approaches, wherein the newly mapped circuitry is modeled, challenged by inducers and perturbed by viral agents, i-FIVE has the potential to promote a systems-level understanding of the interferon branch of molecular innate immunity. This insight may in turn create medical opportunities for the treatment of autoimmune disorders, septic shoc, arthritis as well as in boosting anti-viral responses. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsmedical and health sciencesclinical medicinerheumatologymedical and health sciencesbasic medicineimmunologyautoimmune diseasesmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantivirals Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) ERC-AG-LS2 - ERC Advanced Grant - Genetics, Genomics, Bioinformatics and Systems Biology Call for proposal ERC-2009-AdG See other projects for this call Funding Scheme ERC-AG - ERC Advanced Grant Host institution CEMM - FORSCHUNGSZENTRUM FUER MOLEKULARE MEDIZIN GMBH EU contribution € 1 974 022,40 Address LAZARETTGASSE 14 AKH BT 25.3 1090 Wien Austria See on map Region Ostösterreich Wien Wien Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Gerhard Schadler (Dr.) Principal investigator Giulio Gino Maria Superti Furga (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Beneficiaries (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all CEMM - FORSCHUNGSZENTRUM FUER MOLEKULARE MEDIZIN GMBH Austria EU contribution € 1 974 022,40 Address LAZARETTGASSE 14 AKH BT 25.3 1090 Wien See on map Region Ostösterreich Wien Wien Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Gerhard Schadler (Dr.) Principal investigator Giulio Gino Maria Superti Furga (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data