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Content archived on 2024-06-18

The role of mitotic tissues in controlling the developmental clock

Objective

A number of studies suggest that the larval imaginal discs, the precursors of the adult structures in the fly, play a crucial role in coordinating tissue maturation/patterning with developmental timing. Thus, disc transplantation experiments show that the transplantation of a young imaginal discs into more developmentally advanced larvae delays pupal molt, allowing the underdeveloped discs to catch up with other larval tissues. In contrast, complete ablation of the imaginal discs has no effect on developmental timing of the pupal molt. These results suggest that a signal released from imaginal discs prevents the onset of metamorphosis up until patterning/maturation of the discs is complete. Reminiscent of this, wounding of imaginal discs delays pupal molt, thereby allowing damaged tissue to regenerate before the onset of metamorphosis. So far, the mechanisms by which immature/wounded discs prevent the production of the steroid hormone ecdysone and thereby pupal molt remains elusive. The aim of the proposed project is to screen for the signals that couple patterning of mitotic tissue with the developmental clock using the power of Drosophila genetics. For this purpose we developed two different conditions for which we can induce a developmental delay by altering the expression of two selected genes specifically in the discs. These two conditions will be used at two successive levels to screen the VDRC collection of UAS-RNAi lines (12.000 lines covering 95% of the genes having a mammalian ortholog) for candidates that can revert or at least partially rescue the developmental delay.

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Topic(s)

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Call for proposal

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FP7-PEOPLE-2009-IEF
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
EU contribution
€ 166 145,60
Address
RUE MICHEL ANGE 3
75794 PARIS
France

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Region
Ile-de-France Ile-de-France Paris
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Research Organisations
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Total cost

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