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Content archived on 2024-06-18

The highly calcium-sensitive pool of granules in biphasic insulin secretion: Experimental and theoretical investigations

Objective

Insulin is released from the pancreatic beta-cells in a biphasic manner in response to a step in the ambient glucose concentration, and the loss of first-phase secretion is an early marker of diabetes. Studies have shown that there exist pools of insulin secretory granules with different calcium sensitivities. The project will study the nature, regulation and role of the highly calcium-sensitive pool (HCSP) in pancreatic beta-cells. Special focus will be on its relation to "newcomer granules", which fuse shortly after arriving to the membrane, during biphasic insulin secretion. We will apply a combination of well-established experimental methods such as patch-clamp techniques, life confocal and TIRF imaging, in novel combinations with knock-down of key exocytotic proteins, in particular Syntaxin and Synaptotagmin. The experimental part will be complemented and supported by modelling studies, building on our recently published model, which identified the HCSP with newcomer granules. This interdisciplinary project will test the model experimentally, and vice versa, use the model to suggest key experiments. The suggested work will complement the theoretical training of the fellow with hands-on experience.

Topic(s)

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Call for proposal

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FP7-PEOPLE-2009-IEF
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

MAX IV Laboratory, Lund University
EU contribution
€ 136 002,05
Address
Paradisgatan 5c
22100 LUND
Sweden

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Region
Södra Sverige Sydsverige Skåne län
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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