Objetivo The long-term aim of the proposed study is to resolve the genetic and cell biological mechanisms that contribute to the establishment of vertebrate brain asymmetry. In the zebrafish forebrain, an event crucial in establishing and elaborating asymmetry is the migration of the parapineal primordium. Parapineal cells are specified bilaterally at the midline. They subsequently assemble into a rosette-like structure and migrate to the left side of the brain. The molecular basis of the cellular communication among the parapineal cells during migration is still poorly understood. Preliminary data in Dr Wilson’s group show that Fgf8 induces highly focal pathway activation prior to and during migration. Fgf-reporter activation is found only in one or a few cells that are likely at the leading edge of the migrating primordium. This reporter activation occurs concurrently with suppression of activity in adjacent cells. Our hypothesis is that Fgf pathway activation non-autonomously suppresses pathway activity in neighbouring cells thereby establishing polarity within the entire primordium. In the proposed project, I will utilise multiple techniques to address specific questions with the following immediate aims: 1) to establish how localised activation of Fgf signalling correlates with parapineal migration; 2) to determine how Fgf pathway activation non-autonomously regulates pathway activity in neighbouring cells by addressing the roles of candidate interacting pathways (Notch and Wnt); 3) to determine the molecular mechanisms that govern the cross-talk between Fgf signalling and any of the identified candidates in aim 2. Programa(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) FP7-PEOPLE-2009-IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Convocatoria de propuestas FP7-PEOPLE-2009-IEF Consulte otros proyectos de esta convocatoria Régimen de financiación MC-IEF - Intra-European Fellowships (IEF) Coordinador UNIVERSITY COLLEGE LONDON Aportación de la UE € 181 103,20 Dirección GOWER STREET WC1E 6BT LONDON Ver en el mapa Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Greta Borg-Carbott (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos
