Objective
We propose to solve the crystal structures of the dengue virus (DV) envelope (E) protein from different serotypes in complex with immunologically relevant ligands from the human host.
These will be:
- Dendritic cell-specific ICAM-3 grabbing non-integrin (DC -SIGN). This molecule has been shown to be essential for entry of mosquito-grown virus into human dendritic cells.
- Fab fragments from antibodies capable of neutralising one or more DV serotypes.
In both cases we will be especially interested in serotype de pendent variations and their functional consequences, and will use a comprehensive range of DV field strains isolated from dengue fever patients in Venezuela in an extensive comparative study.
Analysis of the crystal structures will be performed in the con text of binding studies of the ligands with both intact virus and purified E-protein from the corresponding strains. In the case of DC-SIGN the results will be correlated with epidemiological data on the relative virulence of the different serotypes.
This study will elucidate the details of the key molecular interactions at the host cell surface, which occur during the primary DV infection at the site of the mosquito bite. Furthermore, it will reveal the specific antigenic determinants of the E-protein and their serotype dependence, which will be extremely valuable for the development of a safe DV vaccine.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences health sciences infectious diseases RNA viruses
- engineering and technology materials engineering crystals
- natural sciences biological sciences microbiology virology
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs vaccines
- natural sciences biological sciences zoology invertebrate zoology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.