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Content archived on 2024-06-18

Dynamic super-resolution mapping of neurotransmitter release events and synaptic glutamate receptors

Objective

"Proper functioning of the brain requires points of rapid communication between nerve cells. This is achieved at specialized junctions called chemical synapses, which function by the vesicular release of neurotransmitter onto a post-synaptic apposition decorated with neurotransmitter-gated ion channels. Fast chemical synapses release glutamate to activate AMPA-type glutamate receptor ion channels, which excite the post-synaptic neuron. Since these receptors desensitize rapidly, their dynamic exchange with naïve extrasynaptic receptors by surface diffusion plays an important role in shaping the short-term plasticity of synapses and thus the fidelity of synaptic transmission. Localized glutamate transients in the synaptic cleft and the gating kinetics of AMPA receptors occur on the millisecond timescale. Furthermore, the steep glutamate concentration gradients lead to heterogeneous binding-site occupancy and receptor gating. Therefore, the position of vesicle release locations relative to AMPA receptor confinement and diffusion are of crucial importance to understanding the fundamental operating principles of excitatory synapses. Therefore, this project proposes new super-resolution methods for simultaneous live-cell imaging of individual vesicle release events and synaptic AMPA receptor confinement and diffusion. Within this context, the project will also investigate a role in short-term plasticity for multiple release locations and the diffusional exchange of low-occupancy AMPA receptors at the synapse."

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

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FP7-PEOPLE-2010-IEF
See other projects for this call

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
EU contribution
€ 185 748,00
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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