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Content archived on 2024-06-18

Identification of genetic determinants involved in Streptococcus pneumoniae pathogenesis

Objective

Respiratory tract infections are a leading cause of global mortality and morbidity. It has been estimated by the WHO that annually 4-5 million people die of pneumonia. Streptococcus pneumoniae, aptly named because it is the most important bacterial cause of pneumonia, is likely primarily responsible for these deaths. Infection by S. pneumoniae is a complex process dependent on a number of essential pathways, of which members could form ideal candidates for drug design. To identify microbial genes required for pathogenesis, we plan to use an insertion knockout strategy that allows rapid identification of disrupted genes. The host lab successfully performed micro array based approaches and proof-of-concept next generation sequencing methodologies to determine which genes are essential under a number of relevant in vitro and in vivo conditions. To identify shared essential pathways we will use statistical analysis, pathway analysis, network reconstruction and functional category enrichment methods on existing data and data generated within this project to determine the most ideal candidates for drug design. These key genes do not necessarily have to be detected initially in all screenings because of the noise inherent to the techniques used or limited survival of the mutant library. Genes coding for products with the appropriate characteristics will be subsequently tested in an animal model. Vaccines and novel drugs generated from this approach will provide alternatives to continue treatment and prevention of life-threatening chronic and acute bacterial infectious diseases.

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2010-IEF
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

STICHTING RADBOUD UNIVERSITEIT
EU contribution
€ 184 540,80
Address
HOUTLAAN 4
6525 XZ Nijmegen
Netherlands

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Region
Oost-Nederland Gelderland Arnhem/Nijmegen
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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