Objective
Biological redox reactions have been identified as control mechanisms for all aspects of cellular life. Extracellular redox signals contribute to cellular communication, including host-tumour and host-pathogen interactions.
Recent evidence suggests that disulfide bonds in extracellular domains of cell surface receptors have the potential to act as redox-operated switches for protein function. However, target proteins of extracellular redox activity are mostly unknown and the impact of specific cell surface redox changes on cellular interactions and signal transduction remains to be studied.
We propose to identify and functionally analyze redox-regulated surface proteins involved in cellular communication. We will focus on redox signals involved in the activation of primary T cells and in the growth of transformed T cells. Both processes are stimulated by the thiol-disulfide oxidoreductase thioredoxin-1, which is either secreted by dendritic cells in response to antigen-specific recognition of T cells or by T cells themselves as a result of transformation.
The extracellular activities of thioredoxin are known to depend on its redox activity. It is not yet understood how redox changes on the cell surface effect increased sensitivity to particular growth factors and cytokines. In order to identify thioredoxin-regulated proteins on T cells we will apply novel strategies of functional proteomics, including mechanism-based trapping with mutant thioredoxin-1.
Thioredoxin-based signals and their disruption will be analyzed in a DC-T cell coculture system. We are also pioneering techniques to track redox changes by flow cytometry and thereby aim at correlating in vivo events with specific redox signals. We expect to obtain fundamental insight into the emerging field of extracellular redox signalling.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences chemical sciences organic chemistry organic reactions
- natural sciences chemical sciences electrochemistry electrolysis
- natural sciences chemical sciences catalysis
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-8
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
HEIDELBERG
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.