Skip to main content

A new model for study relationship between pluripotency and tumorogenesis: molecular insights from basal chordates

Objective

The potential formation of teratoma/teratocarcinoma from pluripotent stem cells represents one the main obstacles to safe use stem cell-based regenerative therapy. The gene network that regulates the stem cell tumoreginicity is still largely unknown. In this study we use as model the chordate Botryllus schlosseri (Ascidiacea), wherein through a process known as vascular budding, an adult is regenerated from pluripotent cells in a sequence of morphologically abnormal developmental stages which pass through a teratoma structure. The teratoma maintains a population of cells that preserve their pluripotency and eventually re-gain positional identity and differentiate “correctly” into a functional body. In this proposed project we take advantage of this in vivo phenomenon to explore the molecular bases regulating the equilibrium between pluripotency and tumorigenicity. We propose two complementary approaches: (1) To undertake unbiased high-throughout gene screening in order to define the trascriptome implicated in the vascular budding (2) to target candidate genes involved in endomesoderm specification and characterize their spatio-temporal patterns of expression and test their functions.

Field of science

  • /medical and health sciences/medical biotechnology/cells technologies/stem cells

Call for proposal

FP7-PEOPLE-2010-RG
See other projects for this call

Funding Scheme

MC-IRG - International Re-integration Grants (IRG)

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Address
Rue Michel Ange 3
75794 Paris
France
Activity type
Research Organisations
EU contribution
€ 100 000
Administrative Contact
Sophie Deschaintres (Mrs.)