High Throughput Systematic Single Cell Genomics using Micro/Nano-Fluidic Chips for Extracting, Pre-analysing, Selecting and Preparing Sequence-ready DNA

Información del proyecto

CELL-O-MATIC

Identificador del acuerdo de subvención: 278204

Proyecto cerrado

Fecha de inicio 1 Enero 2012

Fecha de finalización 31 Diciembre 2015

Financiado con arreglo a

• Specific Programme "Cooperation": Health

Coste total

€ 8 434 176,51

Aportación de la UE

€ 5 998 788,00

Coordinado por

DANMARKS TEKNISKE UNIVERSITET
Denmark

Objetivo

We propose a technology that will sit at the front-end of sequencing pipelines, present and future, and will significantly enhance the quality and throughput of DNA sequencing.

Although much attention has been given to throughput/cost of the sequencing process itself, the same cannot be said for the preparation of samples. Identified bottlenecks are (1) sequencing technologies require days of upfront sample preparation which is further increased when sequencing selected parts of the genome; (2) genome assembly relies on computationally intensive comparisons to the reference genome because existing technologies produce short sequence reads; (3) it is difficult to begin with small amounts of sample material comprising micro-biopsies and single cells.

The CELL-O-MATIC project will synergize efforts from SMEs, academics and large companies to address these bottlenecks by developing chip-based systems that process DNA from individual cells, ready for next generation high-throughput sequencing.

Single cell analysis has numerous applications in systems biology but we will emphasize DNA isolation and sequencing from circulating tumor cells (CTC), which have a strong prognostic value in cancer management.

A second innovation will be to develop methods that enable up to whole chromosome lengths of DNA to be contiguously mapped using nanofluidics. The inclusion of nanofluidics makes the project particularly distinctive and introduces European SMEs to an area that so far has been the domain of US companies.

A modular prototype comprising, a chip, fluid and thermal control, sonication and optical detection will be developed. Samples prepared using CELL-O-MATIC technology will be benchmarked in a high throughput environment with samples prepared by existing methods. Finally, the information obtained from the CELL-O-MATIC processed sample material will be validated for its utility as an aid to clinical decision making.

Ámbito científico (EuroSciVoc)

• ciencias naturales › ciencias biológicas › genética › ADN
• ciencias médicas y de la salud › medicina clínica › oncología
• ciencias naturales › ciencias biológicas › genética › cromosoma
• ciencias naturales › ciencias biológicas › genética › genoma

Programa(s)

• FP7-HEALTH - Specific Programme "Cooperation": Health

Tema(s)

• HEALTH.2011.1.1-1 - SME-targeted research for developing tools and technologies for high-throughput research

Convocatoria de propuestas

FP7-HEALTH-2011-two-stage
Consulte otros proyectos de esta convocatoria

Régimen de financiación

CP-FP - Small or medium-scale focused research project

Coordinador

DANMARKS TEKNISKE UNIVERSITET

• Aportación de la UE: € 1 710 434,56
• Dirección: ANKER ENGELUNDS VEJ 101 ; 2800 KONGENS LYNGBY ; Dinamarca
• Región: Danmark › Hovedstaden › Københavns omegn
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: Sin datos

Participantes (13)

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD

Reino Unido

• Aportación de la UE: € 588 183,40
• Dirección: WELLINGTON SQUARE UNIVERSITY OFFICES ; OX1 2JD Oxford
• Región: South East (England) › Berkshire, Buckinghamshire and Oxfordshire › Oxfordshire
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: Sin datos

PHILIPS ELECTRONICS NEDERLAND BV

Países Bajos

• Aportación de la UE: € 273 604,00
• Dirección: HIGH TECH CAMPUS 52 ; 5656 AG Eindhoven
• Región: Zuid-Nederland › Noord-Brabant › Zuidoost-Noord-Brabant
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

ROCHE DIAGNOSTICS GMBH

Alemania

• Aportación de la UE: € 12 295,85
• Dirección: Sandhofer Strasse 116 ; 68305 Mannheim
• Región: Baden-Württemberg › Karlsruhe › Mannheim, Stadtkreis
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

GENOTYPE2PHENOTYPE LTD

Reino Unido

• Aportación de la UE: € 333 382,92
• Dirección: ST JUDES CHAMBERS 103A MANNINGHAM LANE ; BD1 3BN BRADFORD
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

DIAGENODE

Bélgica

• Aportación de la UE: € 481 730,17
• Dirección: RUE DU BOIS SAINT JEAN 3 LIEGE PARK SCIENCE ; 4102 Seraing
• Región: Région wallonne › Prov. Liège › Arr. Liège
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

OXFORD NANOPORE TECHNOLOGIES LTD

Reino Unido

• Aportación de la UE: Sin datos
• Dirección: ROBERT ROBINSON AVENUE - OXFORD SCIENCE PARK 4 ; OX4 4GA Oxford
• Región: South East (England) › Berkshire, Buckinghamshire and Oxfordshire › Oxfordshire
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

NIL TECHNOLOGY APS

Dinamarca

• Aportación de la UE: € 526 379,59
• Dirección: HALDOR TOPSOES ALLE 1 ; 2800 KONGENS LYNGBY
• Región: Danmark › Hovedstaden › Københavns omegn
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

BIOCOMPUTING PLATFORMS LTD OY

Finlandia

• Aportación de la UE: € 278 200,00
• Dirección: INNOPOLI 3 VAISALANTIE 6 ; 02130 ESPOO
• Región: Manner-Suomi › Helsinki-Uusimaa › Helsinki-Uusimaa
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

FLUIGENT SA

Francia

• Aportación de la UE: € 227 237,01
• Dirección: 57-77 AVENUE DE FONTAINEBLEAU ; 94270 Le Kremlin-Bicetre
• Región: Ile-de-France › Ile-de-France › Val-de-Marne
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

FASTERIS SA

Suiza

• Aportación de la UE: € 670 600,00
• Dirección: CHEMIN DU PONT DU CENTENAIRE 109 ; 1228 PLAN LES OUATES
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

DNA ELECTRONICS LTD

Reino Unido

• Aportación de la UE: Sin datos
• Dirección: JOHN STREET 10 ; WCIN 2EB LONDON
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

PHILIPS BIOCELL AS

Dinamarca

• Aportación de la UE: € 681 010,00
• Dirección: Gydevang 42 ; 3450 ALLEROD
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos

GENOTYPE2PHENOTYPE LLC

Estados Unidos

• Aportación de la UE: € 215 730,50
• Dirección: 30 ELMWOOD AVE ; 02138 CAMBRIDGE
• Tipo de actividad: Private for-profit entities (excluding Higher or Secondary Education Establishments)
• Coste total: Sin datos