Skip to main content

High-Density Peptide MicroArrays and high-throughput, label-free detection of peptides, modifications and interactions

Objective

Proteins are extremely malleable building blocks of life involved in all aspects of biology. Many diseases are caused by proteins aberrations, and proteins are frequent targets of intervention. Mapping all proteins and their functions are expected to yield pervasive medical and biotechnological benefits. However, even the most comprehensive and high-throughput proteins discovery technologies are seriously challenged by the extreme diversity and low abundance of many proteome components; a problem, compounded by the lack of affinity reagents and validated probes for sample preparation and identification. Our concept is that shorter protein fragments, or peptides, may offer solutions to many of these problems as peptides may represent or mimic proteins. Using in situ solid-phase peptide synthesis, computerized photolithography and novel photochemistry, we have recently generated peptide microarrays of up to 2 million addressable peptides. This unprecedented high-density and high-content peptide microarray technology could make inroads into the kind of high-throughput analysis needed to address the entire human proteome. Here, we aim to exploit this potential by using and improving three different, yet complementary, label-free detection technologies allowing sensitive, high-resolution determinations of the identity, quality and/or modification of individual members of a peptide microarray, and real-time monitoring of any interacting molecular receptor. We will also develop peptides as rapid, specific, and renewable affinity reagents for complex sample preparation, and develop peptides as probes and complex biosensors. Three SME’s constitute the backbone of this collaboration, receiving 50% of the budget, and enjoying significant opportunities from the booming protein/peptide microarray market. Furthermore, solutions to these unmet needs of proteomics are believed to have incalculable benefits for European health, innovativeness and competiveness.

Field of science

  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/proteomics

Call for proposal

FP7-HEALTH-2011-two-stage
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project
Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Coordinator

KOBENHAVNS UNIVERSITET
Address
Norregade 10
1165 Kobenhavn
Denmark
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 717 609,85
Administrative Contact
Ivan Kristoffersen (Mr.)

Participants (9)

SCHAFER -N APS
Denmark
EU contribution
€ 1 061 166,80
Address
Kystvej 6
3050 Humlebaek
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Claus Schafer Nielsen (Dr.)
IMABIOTECH SAS
France
EU contribution
€ 658 300
Address
152 Rue Du Docteur Yersin
59120 Loos
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Jonathan Stauber (Dr.)
PLASMORE SRL
Italy
EU contribution
€ 697 860
Address
Viale Vittorio Emanuele Ii 4
27100 Pavia
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Andrea Valsesia (Dr.)
JRC -JOINT RESEARCH CENTRE- EUROPEAN COMMISSION
Belgium
EU contribution
€ 316 510,80
Address
Rue De La Loi 200
1049 Brussels
Activity type
Research Organisations
Administrative Contact
Ian Formosa (Dr.)
FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
Germany
EU contribution
€ 147 831
Address
Hansastrasse 27C
80686 Munchen
Activity type
Research Organisations
Administrative Contact
Walter Krause (Mr.)
BRUKER DALTONIK GMBH
Germany
EU contribution
€ 148 817,50
Address
Fahrenheitstrasse 4
28359 Bremen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Tanja Lorek (Ms.)
THE UNIVERSITY OF MANCHESTER
United Kingdom
EU contribution
€ 314 070
Address
Oxford Road
M13 9PL Manchester
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Liz Fay (Ms.)
UNIVERSITY OF BATH
United Kingdom
EU contribution
€ 444 300,80
Address
Claverton Down
BA2 7AY Bath
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Hazel Wallis (Ms.)
DANMARKS TEKNISKE UNIVERSITET
Denmark
EU contribution
€ 316 547,25
Address
Anker Engelundsvej 1 Bygning 101 A
2800 Kgs Lyngby
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jesper Thorsgaard (Mr.)