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New tools for genetic engineering using targeted integration vectors application to agronomy food safety and gene therapy

Objective

The overall objectives of the INTEGRA project is to develop new genetic engineering tools that will permit targeted integration of a transgene into the genome of the desired eukaryotic organism. This project will allow the easy and rapid generation of transgenic knock out or knock in small and large animals without the need of embryonic stem cells. This will have visible repercussions on fundamental and applied research particularly in pharmaceutics agronomy and food sciences.

At the same time it will strengthen European research in the field of gene therapy with the development of safe viral vectors based on non integrative or targeted integration vectors adequate for clinical use. We plan to meet the objectives by two alternative means. The first one will combine the use of a lentiviral episomal vector with a phage site specific integration system to direct the integration of the transgene into a unique locus in the host genome. The alternative method will use lentiviral vectors for which the integrase specificity will be modified HIV integrases will be generated whose DNA binding domain will be replaced by a synthetic DNA binding domain able to recognize any given DNA sequence of 9 or 18 base pairs. Such specific integrase will be incorporated into a gene transfer vector system that will allow the genetic modification of somatic cells and zygotes.

These technologies will be used by the partners to generate transgenic livestock with modified traits improved growth traits disease resistance and to generate sensor animals to monitor contamination of livestock by toxic agents. On the other hand gene therapy preclinical studies will be performed using the secured vectors Specific ethical and societal implications of the project will be anticipated through the participation of European stakeholders. The overall INTEGRA project clearly cuts across the Thematic Priority 1 and 5 and the Science and Society programme of FP6.

Field of science

  • /agricultural sciences/agriculture, forestry, and fisheries/agriculture/agronomy

Call for proposal

FP6-2004-NEST-C-1
See other projects for this call

Funding Scheme

STREP - Specific Targeted Research Project

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Address
3 Rue Michel Ange
Paris
France

Participants (8)

CENTRE DE REGULACIO GENOMICA
Spain
Address
Passeig Maritim, 37-49
Barcelona
ROSLIN INSTITUTE
United Kingdom
Address
Roslin Midlothian
Roslin
THE UNIVERSITY OF COPENHAGEN
Denmark
Address
Norregade 10
Copenhagen
FONDATION ASILE DES AVEUGLES
Switzerland
Address
15, Av. De France
Lausanne
THE UNIVERSITY OF EDINBURGH
United Kingdom
Address
Old College, South Bridge
Edinburgh
INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE
France
Address
101 Rue De Tolbiac
Paris
UNIVERSITÉ DE STRASBOURG
France
Address
Blaise Pascal 4
Strasbourg
UNIVERSITÉ PARIS SUD XI
France
Address
15 Rue Georges Clémenceau
Orsay