Objetivo The primary aims of this work are tightly connected: (i) development of a bio-inspired “synthetic cell” capable of self-assembling, self-propelling and environmental sensing prepared with reduced molecular complexity compared to living cells (ii) quantitative assessment of the bio-activity of specific cellular components within these “synthetic cells”, leading to better fundamental understanding of their function in living cells (iii) use of these findings for reverse engineering of living cells with tailored adhesive and sensory properties.Integrin based adhesion has been shown to participate in numerous processes in living cells, which sense, via their adhesions, multiple environmental cues, integrate them, and develop a complex, multi-parametric response. However, due to their intrinsic molecular complexity the specific functional roles of different components of the adhesion site are still poorly understood. To address this issue, we will utilize current knowledge of the modular nature of focal adhesions and related integrin-mediated extracellular matrix contacts to develop “synthetic cell” models, consisting of large lipid vesicles, functionalized by transmembrane integrins, various integrin-binding proteins and specific sets of scaffolding and signaling proteins of the adhesion sites. The one-by-one loading of these vesicles by micro-injection with these proteins will allow tight control of the system composition and complexity, and testing of the effect of compositional and environmental variations on the adhesion and signaling features. The “synthetic cells” will be plated on adhesive matrices displaying specific spatial, chemical and mechanical features for testing their chemical and mechanical sensing capabilities. The datasets produced in these experiments will provide a solid basis for reverse engineering perturbations of living cells, in which specific functional pathways will be targeted and/or modified to modulate living cells' functionality. Ámbito científico natural sciencesbiological sciencessynthetic biologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesbiochemistrybiomoleculeslipids Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-AG-LS9 - ERC Advanced Grant - Applied life sciences and biotechnology Convocatoria de propuestas ERC-2011-ADG_20110310 Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-AG - ERC Advanced Grant Institución de acogida MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Aportación de la UE € 1 749 800,00 Dirección HOFGARTENSTRASSE 8 80539 Munchen Alemania Ver en el mapa Región Bayern Oberbayern München, Kreisfreie Stadt Tipo de actividad Research Organisations Contacto administrativo Richard Segar (Dr.) Investigador principal Joachim Spatz (Prof.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Alemania Aportación de la UE € 1 749 800,00 Dirección HOFGARTENSTRASSE 8 80539 Munchen Ver en el mapa Región Bayern Oberbayern München, Kreisfreie Stadt Tipo de actividad Research Organisations Contacto administrativo Richard Segar (Dr.) Investigador principal Joachim Spatz (Prof.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos WEIZMANN INSTITUTE OF SCIENCE Israel Aportación de la UE € 1 749 999,00 Dirección HERZL STREET 234 7610001 Rehovot Ver en el mapa Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Gabi Bernstein (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos
