Logging out of EU Login will log you out of any other services that use your EU Login account. Use the CORDIS log out button to remain logged in on other services.
This is a machine translation provided by the European Commission’s eTranslation service to help you understand this page. Please read the conditions of use.
Engineering of an Artificial Capsidic Enzyme for Aqueous Dirhodium Catalysis
Final Report Summary - ACCARC (Engineering of an Artificial Capsidic Enzyme for Aqueous Dirhodium Catalysis)
Supercharging can endow a protein scaffold with new properties. By computational design, the surface of the protein nanocage ferritin was reengineered to produce a supercharged nanocage. The supercharged ferritin binds to negatively charged surfaces, and retains high thermostability. Synthesis of iron-oxide nanoparticles enables monitoring of the spatial localization of the nanocages in transfection experiments. Supercharged ferritin is readily incorporated into cells of a human cancer cell model. By tuning the electrostatic interactions between supercharged ferritin and a larger redesigned protein nanocage (AaLS 13) nested, Matryoshka-type structures were obtained, with several iron loaded, supercharged ferritin nanocages encapsulated within the larger cage. These experiments open up new avenues towards the construction of nano-sized structures such as artificial organelles or microcompartments.