Objective
As global food prices mount, the imperative for novel approaches to crop growth becomes evident. A recently discovered group of glycosylated peptides with hormonal characteristics has a similar impact on plant growth and environmental response as the classical phytohormones that were pivotal in the first green revolution.
We aim to develop methods for the in-vitro synthesis of these hormones.
Glycosylation is essential for peptide function. Identification of glycosyl transferases (GTs) acting on these peptides is a prerequisite for in-vitro synthesis. I will identify potential GTs by combining existing bioinformatic techniques and genomic data available at the host institution with proteomic data that I bring with me from my previous post-doctoral position in the US. I will also search for the last unidentified GT initiating glycosylation in a related, non-hormonal group of secreted peptides.
Working with molecular biologists at the host institution, I will develop my molecular skills constructing tagged synthetic peptides and expressing these in putative GT mutants selected from the proteomic and genomic data. Working with mass spectrometry specialists I will analyse glycosylation signatures on peptides retrieved from wild type and mutant plants.
Simultaneously, the fate of synthetic peptides progressing through the secretory system will be followed using protocols I developed during my time in the US and equipment that I bring with me. This will establish the distribution of GTs throughout the secretory system and act as a proof of concept for this technique in analysing modifications on other secreted proteins.
Putative GTs expressed in heterologous systems with specific donor and acceptor substrates will confirm GT identity and demonstrate in-vitro synthetic capacity, a move towards developing novel agricultural tools.
This project will develop state-of-the-art methods with multiple applications and mark a EU contribution to an exciting new field.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- social sciences political sciences political transitions revolutions
- agricultural sciences agriculture, forestry, and fisheries agriculture
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
1165 KOBENHAVN
Denmark
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.