Final Report Summary - MEM-STRUCT-AFM (The Structure and Assembly of Membrane Proteins in Native Membranes studied by AFM) High-Speed Atomic Force Microscopy: The dawn of dynamic structural biochemistry:The advent of high-speed atomic force microscopy (HS-AFM1) has opened a novel research field for the dynamic analysis of single bio-molecules: Molecular motor dynamics2,3 membrane protein diffusion4,assembly5 and conformational changes6 could be directly visualized. Further developments for buffer exchange7 and temperature control8 during HS-AFM operation provide breakthroughs towards the performance of dynamic structural biochemistry using HS-AFM.1) Ando et al, Chem Rev 2014, 114(6):3120-882) Kodera et al, Nature 2010, 468(7320):72-63) Uchihashi et al, Science 2011, 333(6043):755-84) Casuso et al, Nat Nanotechnol 2012, 7(8):525-95) Chiaruttini et al, Cell 2015, 163(4):866-79.6) Ruan et al, PNAS 2017, doi: 10.1073/pnas.16164131147) Miyagi et al, Nat Nanotechnol 2016, 11(9):783-908) Takahashi et al, Small 2016, 12(44):6106-6113
