Cryptochromes are flavin- and folate-containing proteins with a high degree of similarity to DNA photolyase, which repairs UV-induced DNA damage. Cryptochrome play essential roles in the maintenance of circadian rhythms in mice and human. A transcription-translational feedback loop has been shown to be essential component of clock, and this mechanism seems to be conserved over a wide range of species. The transcriptional activation by CLOCK: BMAL1 heterodimer and inhibition by Cryptochrome and PERIOD are believed to provide the framework of the feedback loop in mammals. With this project interacting domain of mammalian cryptochrome with clock proteins will be identified by mammalian two-hybrid system and co-immuno precipitation assay.
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