Objective
Changes in chromatin structure are observed during aging and are often a critical step during tumorigenesis. The class of long intergenic non-coding RNAs (lincRNA), has emerged as an important regulator of chromatin structure during normal differentiation, and recently has been implicated in the disregulation of chromatin structure observed in several solid tumours. These lincRNAs could represent an important new class of oncogene. We propose to investigate a potential involvement of lincRNAs in chromatin changes that occur during normal aging of haematopoietic stem cells (HSC), and in acute myeloid leukaemia (AML), an age-related haematological malignancy that is frequently associated with chromatin abnormalities. High-throughput sequencing will be used to compare expression of lincRNAs between young and old HSC, and between AML and normal CD34+ cells. Differentially expressed lincRNAs will be functionally investigated by lentiviral-mediated knockdown and overexpression in cell lines and primary cells, and concomitant changes in chromatin structure will be profiled by chromatin immunoprecipitation. It is expected that this project will clarify the general importance of lincRNAs both in normal aging and in an age-related cancer, and could identify novel molecules which are important drivers of malignancy as well as the functional changes associated with HSC aging. As well as being potential prognostic markers and direct targets for therapeutics in AML, these molecules might also be targeted to improve the function of aging HSCs.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology cells technologies stem cells
- natural sciences biological sciences genetics RNA
- medical and health sciences clinical medicine oncology leukemia
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2012-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
F-75205 / 13 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.