Objective
Modification of cells is a central topic in pharmaceutical and medical sciences as well as in basic research. Innumerable opportunities, e.g. in discovery of new regulatory pathways, novel targets, and potential drug candidates as well as clarification of signal transduction are addressed by cell transfection technology. The dominant consideration for cell manipulation is the type of cells used. Until now, most often conventional, immortal cell lines, being cultured for years or even decades in flasks, have been assigned to this specified use, although they are mainly irrelevant in physiological and medical respects. The optimal choice would be primary cells, since they are distinctly closer to the physiological situation than cell lines. Researchers would made rapid strides in the analysis of the biology of cells among others for the development of therapeutics, and new types of treatments as well as possible cures for a variety of diseases and injuries would be possible by using primary cells instead of cell lines. However, primary cells until recently were hard or often even impossible to transfect by non-viral methods, and consequently, many researchers still hesitate to adopt these cells despite their unquestionable advantages. With the advent of the nucleofection technology, a unique method for the highly efficient transfection of primary cells was recently made available to the life science community.
With MODEST the main objectives are i) the development of devices for ultrahigh-throughput nucleofection of primary cells in modular multi-well plates, and ii) the application of this technology to the highly relevant area of immunological as well as neuronal disorders to facilitate investigations of possible mechanisms of intervention.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
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Topic(s)
Call for proposal
FP6-2005-LIFESCIHEALTH-7
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Funding Scheme
STREP - Specific Targeted Research ProjectCoordinator
COLOGNE
Germany