Objective This proposal combines the accumulated expertise from a previous FP5 EU project, Mechanisms of Proteinuria (QLG1-2000-00691) and an ongoing FP6 project, ADDNET (LSHB-CT-2003-503 364). We will utilize the latest knowledge on the pathophysiology of diabetic nephropathy and newly identified urinary markers of diabetic kidney damage to develop a predictive diagnostic test to follow disease progression. Biomarkers identified, in particular those preceding the development of diabetic kidney damage during the pre-microalbuminuric stage, will be evaluated in prospective clinical studies. By metabolomics we aim to find additional markers from diabetic urine, and will identify antibodies recognizing these metabolites/peptides from an antibody phage display-library. In parallel, two separate approaches will be used to develop diagnostic tests, one based on nanobead technology and the other on a multiplexing platform allowing combination of several measurables into a single test. Both technologies will utilize antibody capture methods. This will translate into early identification of patients at high risk of rapid loss of kidney function. The validation of the diagnostic tests emerges from the use of the largest European urine, serum and DNA databases of diabetic nephropathy with meticulously collected follow-up samples. Subsequent steps of the test development include premarketing evaluation and transfer of the test into patient use by an SME of diagnostic service provider in Finland. Another SME will provide market and competitor analyses and search for best market channels for the test in international markets. This approach directly aiming at developing a clinical urinary test will be supported by extensive basic research on the mechanisms/biomarkers of diabetic nephropathy at the level of the kidney. These include a novel method of in vivo biotinylation and state of the art proteomics on kidney samples during the development of experimental diabetic nephropathy. Fields of science medical and health sciencesclinical medicineendocrinologydiabetesdiabetic nephropathynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesmicrobiologyvirologymedical and health sciencesbasic medicinephysiologypathophysiologyengineering and technologynanotechnology Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) LSH-2005-1.2.2-4 - Development of new diagnostics LIFESCIHEALTH - Life sciences, genomics and biotechnology for health Call for proposal FP6-2005-LIFESCIHEALTH-7 See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator HELSINGIN YLIOPISTO EU contribution No data Address Yliopistonkatu 4 UNIVERSITY OF HELSINKI, HELSINKI Finland See on map Links Website Opens in new window Total cost No data Participants (8) Sort alphabetically Sort by EU Contribution Expand all Collapse all UNIVERSITEIT LEIDEN Netherlands EU contribution No data Address Rapenburg 70 9500 LEIDEN See on map Links Website Opens in new window Total cost No data PHILOGEN S.P.A. Italy EU contribution No data Address La Lizza 7 SIENA See on map Links Website Opens in new window Total cost No data PHILIPS ELECTRONICS NEDERLAND B.V. Netherlands EU contribution No data Address BOSCHDIJK 525 5621 JG EINDHOVEN See on map Total cost No data FUTURE DIAGNOSTICS BV Netherlands EU contribution No data Address Nieuweweg 172 WIJCHEN See on map Links Website Opens in new window Total cost No data UNITED LABORATORIES LTD Finland EU contribution No data Address Höyläämötie 14 222 HELSINKI See on map Links Website Opens in new window Total cost No data UNITED MEDIX LABORATORIES LTD. Finland EU contribution No data Address Nihtisillankuja 1 ESPOO See on map Links Website Opens in new window Total cost No data EIDGENÖSSISCHE TECHNISCHE HOCHSCHULE ZÜRICH Switzerland EU contribution No data Address Raemistrasse 101 ZURICH See on map Links Website Opens in new window Total cost No data SAMFUNDET FOLKHÄLSAN I SVENSKA FINLAND R.F. Finland EU contribution No data Address Topeliuksenkatu 20 211 HELSINKI See on map Links Website Opens in new window Total cost No data