Objectif Xylem is the vascular tissue responsible for conducting water and minerals between roots and leaves. The xylem vessels are made of conducting cells called tracheary elements (TEs).Before these cells undergo programmed cell death and the end walls dissolve to form a hollow tube, the cortical microtubules (MTs) bunch together to form transverse hoops and helices that act as a template for the cell wall thickenings that will prevent the hollow xylem from collapsing.I will investigate the proteins involved in the key MT-bunching phase. The formation of TEs and their typically sculptured walls has been studied in differentiating cell cultures mainly mesophyll cells isolated from leaves of Zinnia elegans that transdifferentiate into TEs.More recently, however, Arabidopsis thaliana suspension cultures have been induced to form TEs and this exciting development allows the Arabidopsis genome to be exploited for studying proteins involved in TE differentiation.The applicant has visited/worked in the Japanese labs that pioneered this technique and now proposes a logical extension to his training by working in a lab that has developed methods for the proteomic identification of microtubule-associated proteins (MAPs) from Arabidopsis.I will extend my experience on the Zinnia TE differentiation system to the published Arabidopsis suspension system and will use two complementary approaches to gain expertise on the plant cytoskeleton. I will identify those MAPs that are upregulated during TE differentiation using the proteomic strategy developed in the host laboratory on undifferentiated Arabidopsis cells.I will also examine the role of specific MAPs (including one novel MAP family) already identified in the host laboratory. Targeted proteins will be analyzed during TE differentiation by gene expression and localization studies using antibodies and GFP fusion proteins. Champ scientifique sciences naturellessciences biologiquesbiochimiebiomoléculeprotéinessciences naturellessciences chimiquesscience des polymèressciences naturellessciences biologiquesgénétiquegénomesciences agricolesagriculture, sylviculture et pêchesylviculture Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Appel à propositions FP6-2005-MOBILITY-5 Voir d’autres projets de cet appel Régime de financement EIF - Marie Curie actions-Intra-European Fellowships Coordinateur JOHN INNES CENTRE Adresse Norwich research park, colney Norwich Royaume-Uni Voir sur la carte Liens Site web Opens in new window Contribution de l’UE Aucune donnée
