Objective
The objective of this proposal is to investigate the transcriptional regulation of genes encoding the antioxidant network and their cognate transcriptional factors (TFs) in bundle sheath cells (BSC) of the Arabidopsis leaf.
Based on previous data concerning the BSC-specific stress-responsive expression of the ascorbate peroxidase 2 gene (APX2), it is postulated that the expression of the antioxidant defence gene network and its controlling TFs in BSC of the leaf subject to excess light stress, wounding or pathogen infection is constituted differently from that in cells of the leaf lamina.
Therefore, it is postulated that a regulatory network model, and by implication its description of cellular ROS metabolism, will have different properties to those derived for the whole leaf. I propose to establish a novel technique based on epitope-tagging of ribosomes only in a particular cell type and then to purify RNA specific to BSC and lamina cells separately from selectively immunoprecipitated polyribosomes. Next, the expression of antioxidant genes and TF genes known to influence their function will be examined by qRT-PCR.
Finally, computational models will be developed to determine the likely impact of the stresses on antioxidant and ROS metabolism in these cells compared with that from lamina tissue. These results will contribute to the development of sustainable agricultural systems since the knowledge of antioxidant networks in specific tissues will allow to determinate the most favourable conditions for the most efficient utilisation of environmental resources and therefore reduce chemical pesticides in crop production.
It will also contribute to other fields, even biomedical, since the technology of epitope-tagging of ribosomes to purify RNA from a specific cell type potentially is universal and, it could be used to decipher the function of genes and gene products relevant to human health and to explore their interactions with each other and with their environment.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- agricultural sciences agriculture, forestry, and fisheries agriculture sustainable agriculture
- natural sciences biological sciences biochemistry biomolecules proteins
- agricultural sciences agriculture, forestry, and fisheries agriculture agronomy
- natural sciences biological sciences genetics RNA
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
COLCHESTER
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.