Objective
Ldb1, a ubiquitously expressed LIM domain binding protein, is essential in a number of tissues during development. It interacts with Gata1, Tal1, E2A and Lmo2 to form a transcription factor complex regulating late erythroid genes.
It has been identified by the host laboratory a number of novel Ldb1 interacting proteins in erythroleukemic cells, in particular the repressor protein Eto2 (and its family member Mtgr1) and the cyclin dependent kinase Cdk9. Morpholino mediated knockdowns in zebrafish show these f actors to be essential for definitive hematopoiesis. In accordance with the zebrafish results these factors are co-expressed in pre-hematopoietic cells of the early mouse embryo although the complexes were originally identified in late erythroid cells.
Based on the change in subcellular localisation of Eto2, we postulate that it plays a central role in the transition from the migration and expansion phase of the pre-heamatopoietic cells to the establishment of definitive hematopoietic stem cells. My project will focus on the understanding of the role of the two newly identified Ldb1 binding partners, (Eto2 and Cdk9) in the regulation of the formation of the blood system.
In order to achieve my goals, I will use a multidisciplinary approach involving affinity tagging of the proteins (allowing purification and mass spectrometry identification of interacting partners), functional testing of the novel partner proteins in zebrafish, and mouse genetic modification to obtain in vivo data through conditional knockouts.
The role of Eto2 and Cdk9 will be studied at the cellular and organism level. I will use mice and cells to identify their target genes and to gain insight into their role in the development of stem cells and erythroid differentiation in vivo.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences medical biotechnology genetic engineering
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences medical biotechnology cells technologies stem cells
- medical and health sciences clinical medicine embryology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
ROTTERDAM
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.