Objective
Optical imaging techniques and probe development have boosted knowledge on genomics and proteomics. High quality imaging techniques and novel sources of contrast are being developed, however 3D information and more importantly quantification is still lacking especially at a resolution higher than that provided by confocal microscopy. It is now high time to develop more elaborate approaches that will improve resolution, sensitivity and specificity of real-time in-vivo imaging of gene expression and biological processes in 3D.
We propose to develop a novel microscopy technique based on tomographic approaches for 3D in-vivo imaging of biological processes. Optical tomography provides isotropic optical resolution in all three dimensions of space; this resolution is however limited by diffraction to about 250nm for visible light. To overcome this impasse, structured illumination will be implemented, thus improving the resolution drastically compared to conventional microscopy techniques. By making use of saturate d patterned fluorescence excitation, the resolution barrier can be overcome altogether.
This technique will be applied to imaging structure and dynamics at the cell nuclear level. Subsequently its potential has to be tested in the analysis of complex systems such as the drosophila melanogaster. To that end, a custom-made microscope will be extended for structured and saturated fluorescence excitation, and corresponding inverse mapping algorithms will be developed to reconstruct the objects or the fluorescence dye distribution respectively from the 3D images. This new optical imaging device will then be used to quantitatively study nuclear and sub-cellular structure, organisation and dynamics in 3D.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences data science
- natural sciences biological sciences genetics
- natural sciences computer and information sciences software
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences physical sciences optics microscopy confocal microscopy
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
HERAKLION, CRETE
Greece
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.