Final Activity Report Summary - BRC (Biological Research Center, Hungarian Academy of Sciences)
We developed a method for detection of disease-associated prion protein forms in blood of TSE-affected animals without using protease digestion. The method has been successfully applied to hamster and sheep plasma samples. Contrast to other published approach, our method explores only a simple ELISA step and offers all the advantages (sensitivity, reproducibility, specificity, rapidity, practicability for routine use, amenability to automatisation) known to be associated with an ELISA-based method.