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Content archived on 2024-05-29

Molecular and metabolic effects of transgene insertion in plants and response to abiotic stress

Objective

The use of genetically modified plants raises a number of scientific issues as the evaluation of modifications at genetic and metabolic level induced by the transformation. The glucocorticoid receptor (GR) is a hormone-receptor transcription factor in vertebrates that is activated by gluco-corticoids, having anti-inflammatory and immunosuppressive benefits. Transformants of Nicotiana langsdorffii plants containing the gr gene showed alterations in the morphology and development.

A previous analysis allowed the detection of differences in the transgenes copy number but sequence homology of the transgene insertion sites. Detailed analysis suggested that the insertion of gr in the genome of Nicotiana led to qualitative differences in DNA methylation patterns that are known to regulate transcriptional activity and development. In order to better understand the effects of gr transformation in Nicotiana plants, the METRIC project aims to perform a comprehensive analysis at molecular and metabolic level of the different transformants.

At the same time, plants will be exposed to environmental stress using as example heavy metals with different physiochemical property and functions. This approach will allow a preliminary understanding of the molecular and metabolic effects of the transgene insertion and its role in plant-abiotic stress interaction, gainful goals not only for the biotechnology scientific community but also for all the scientists of related fields, including plant-abiotic stress interactions. The main objective of METRIC is to reintroduce the candidate in multidisciplinary high quality scientific laboratories of the country of origin, giving training in several technical skills and benefiting of the experience acquired during the previous Marie Curie fellowship. The topic of METRIC is specifically relevant to the FP6 scientific thematic priorities "Life Sciences, genomics and biotechnology for health" and "Sustainable development, global change and ecosystems".

Fields of science (EuroSciVoc)

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Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

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FP6-2004-MOBILITY-11
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

ERG - Marie Curie actions-European Re-integration Grants

Coordinator

UNIVERSITY OF FLORENCE, ITALY, DEPARTMENT OF ANIMAL BIOLOGY AND GENETICS LEO PARDI ,
EU contribution
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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