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Content archived on 2024-05-29

Beetle comparative Genomics and Speciation (BUGS): Genomic tools for the analysis of chromosome changes in the evolution of Coleoptera

Objective

The success of the Genome Projects carried out on a handful of model organisms has anticipated the extreme value of genomic comparative analyses as a way to unveil many functions and properties of these genomes. These analyses, anchored on an evolutionary framework, have the potential to explain the value of observed genomic differences in phenotype changes, with implications in research areas from biodiversity and speciation to health issues. However, given the unfeasibility to accomplish full genome studies in most or a good representation of life forms, alternative short-cut strategies have to be devised to grant access to genomic information of a wide variety of organisms and to assess the impact of their differences.

In this project, we propose the combined use of a PCR-based approach and Molecular Cytogenetics techniques (including FISH, M-FISH and Zoo-FISH), coupled with the ability to isolate specific chromosomes using laser micro-dissection techniques, to generate high-resolution physical chromosome maps of selected organisms using genetic landmarks provided by cDNA libraries. These physical maps will be analysed comparatively to trace genomic structural changes over evolutionary periods. We will implement this methodology in the study of speciation and diversification processes in leaf beetles (Chrysomelidae) focusing in the analysis of the genomic changes affecting chromosome structure at three different levels:
- role and constraints of hybridisation as a mechanism for speciation,
- chromosomal changes accompanying a species radiation, and,
- analysis of chromosome synteny in phytophagous Coleoptera.

Fields of science (EuroSciVoc)

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Keywords

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Topic(s)

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Call for proposal

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FP6-2002-MOBILITY-11
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Funding Scheme

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EIF - Marie Curie actions-Intra-European Fellowships

Coordinator

UNIVERSIDAD DE MURCIA
EU contribution
No data
Address
Avda. Teniente Flomesta 5
MURCIA
Spain

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Total cost

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