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Content archived on 2024-06-16

Identification of proteins that confer mRNA identity to RNAs

Objective

Different classes of RNA are exported from the nucleus by distinct transport pathways, and the pathway employed is critical for the cytoplasmic fate of the RNA. It is not well understood how the nuclear export machinery of higher eukaryotes discriminates between the different RNAs, most critically, between messengerRNA (mRNA) and U-rich small nuclear RNAs (U snRNAs) that share similarity but use completely distinct export pathways. The project proposed here aims to identify proteins that restrict an RNA to the mRNA export pathway. Modified forms of the Ul snRNA which are transported like mRNA, will be used to characterise those identity elements. I want to purify the ribonucleoprotein complexes (RNPs) containing the modified Ul snRNAs, identify the proteins attached to the RNA via a proteomic approach and analyse their function in RNP assembly and RNA transport. Furthermore, I intend to investigate how the proteins conferring mRNA identity impair the accessibility of the RNA to other RNA export pathways.

Fields of science (EuroSciVoc)

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Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP6-2002-MOBILITY-5
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

EIF - Marie Curie actions-Intra-European Fellowships

Coordinator

EUROPEAN MOLECULAR BIOLOGY LABORATORY
EU contribution
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Address
Meyerhofstrasse 1
HEIDELBERG
Germany

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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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