Objectif Different classes of RNA are exported from the nucleus by distinct transport pathways, and the pathway employed is critical for the cytoplasmic fate of the RNA. It is not well understood how the nuclear export machinery of higher eukaryotes discriminates between the different RNAs, most critically, between messengerRNA (mRNA) and U-rich small nuclear RNAs (U snRNAs) that share similarity but use completely distinct export pathways. The project proposed here aims to identify proteins that restrict an RNA to the mRNA export pathway. Modified forms of the Ul snRNA which are transported like mRNA, will be used to characterise those identity elements. I want to purify the ribonucleoprotein complexes (RNPs) containing the modified Ul snRNAs, identify the proteins attached to the RNA via a proteomic approach and analyse their function in RNP assembly and RNA transport. Furthermore, I intend to investigate how the proteins conferring mRNA identity impair the accessibility of the RNA to other RNA export pathways. Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsRNA Mots‑clés RNA export nuclear transport proteins Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Appel à propositions FP6-2002-MOBILITY-5 Voir d’autres projets de cet appel Régime de financement EIF - Marie Curie actions-Intra-European Fellowships Coordinateur EUROPEAN MOLECULAR BIOLOGY LABORATORY Contribution de l’UE Aucune donnée Adresse Meyerhofstrasse 1 HEIDELBERG Allemagne Voir sur la carte Coût total Aucune donnée